Purification of Vaccine Candidates Isolated from Streptococcus mutans
نویسندگان
چکیده
INTRODUCTION Endocarditis is a disease where the inner tissue around heart valves becomes inflamed due to a bacterial infection that originates in the bloodstream. It is commonly known that the bacteria from the genus Streptococcus can initiate infection (Moreillon & Que, 2004). No vaccine against streptococcal endocarditis is currently available (Moreillon & Que). Despite lack of success in vaccine development there is a new reason for optimism: a recent achievement in immunology called reverse vaccinology (Rappuoli, 2001). This strategy uses a combination of in silico, in vitro and in vivo techniques and is an efficient way to uncover and to assess universal vaccine candidates. Reverse vaccinology has been defined as a method of producing a vaccine by first studying the genomic information of the organism (in silico) to determine which peptides are most likely to be antigenic (Rappuoli). These predicted peptides will be highly expressed (in vitro) and compiled to represent all the virulent species of Streptococcus. Animal testing of the vaccine candidates will confirm whether or not the selected peptides stimulated an immune response in the host (in vivo). By using reverse vaccinology researchers are able to identify vaccine candidates and subsequently test them for effectiveness across all strains of Streptococcus (Barocchi, Censini, & Rappuoli, 2007). In collaboration with others, this particular project will focus on Streptococcus mutans in order to deduce a universal vaccine for streptococcal endocarditis.
منابع مشابه
The Comparison of Proteins Elaborated by Streptococcus mutans Strains Isolated from Caries Free and Susceptible Subjects
متن کامل
Effect of Isolated Specific Lytic Phage against Growth and Biofilm Inhibition of Streptococcus mutans and Streptococcus sanguinis Isolated from Decayed Dental Plaque
Background and purpose: Despite advances in oral health and dental industry, tooth decay remains one of the most common oral diseases. One of the new methods to combat dental plaque, which is the main cause of caries, is using specific lytic bacteriophage. This study aimed to investigate the effect of isolated specific lytic phage against growth and biofilm inhibition of Streptococcus mutans an...
متن کاملReverse Vaccinology: Combating Endocarditis Caused by Streptococcus mutans
INTRODUCTION Endocarditis is a disease where the inner tissue around heart valves becomes inflamed due to a bacterial infection that originates in the bloodstream. It is commonly known that the bacteria from the genus Streptococcus can initiate infection (Moreillon & Que, 2004). No vaccine against streptococcal endocarditis is currently available (Moreillon & Que, 2004). Despite lack of success...
متن کاملThe Frequency of Streptococcus Mutans and Lactobacillus spp.in 3-5-year- old Children with and without Dental Caries
Abstract Background and Objective: The high occurrence of early childhood caries (ECC) is one of the most common problems in children dentistry. Streptococcus mutans and lactobacilli.spp are closely associated with the development of early childhood caries (ECC). Therefore, the aim of this study was to investigate the frequency of streptococcus mutans and lactobacilli.spp in 3-5 –year- old ch...
متن کاملControl of growth Streptococcus mutans isolated from saliva and dental caries
Streptococcus mutans is a Gram-positive, non-motile, non-spore forming, catalasenegative, facultative anaerobic cocci bacterium commonly found in the human oral cavity, is a significant contributor to tooth decay (David et al., 2011). The mutans streptococci comprise a group of seven species, of which Streptococcus mutans and Streptococcus sobrinus are the predominant species isolated from huma...
متن کامل